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Test

Internal Control

Abbreviations

Pathogen Category

Target Gene

Sample Type

RequiredFluorescenceChannels

Minimum Sensitivity (Copies/Ul)

African Swine Fever Virus

Swine endogenous or exogenous

ASFV

DNA virus

VP72 gene

Oral and nasal swabs, whole blood, tissue, saliva, feed, sewage, excreta and other samples

FAM; VIC

1

African Swine Fever Virus P72 Gene and CD2V Gene

Swine endogenous or exogenous

P72+CD2V

DNA virus

P72+CD2V gene

Oral and nasal swabs, whole blood, tissue, saliva, feed, sewage, excreta and other samples

FAM; VIC; ROX

1

African Swine Fever Virus P72 Gene, CD2V Gene and MGF360-505R Gene

Swine endogenous or exogenous

P72+CD2V+MGF360-505R

DNA virus

P72+CD2V+MGF360-505R gene

Oral and nasal swabs, whole blood, tissue, saliva, feed, sewage, excreta and other samples

FAM; VIC; CY5; ROX

1

African Swine Fever Virus CD2V Gene and MGF360-505R Gene

N/A

CD2V+MGF360-505R

DNA virus

CD2V+MGF360-505R gene

Oral and nasal swabs, whole blood, tissue, saliva, feed, sewage, excreta and other samples

FAM; VIC

1

Porcine Epidemic Diarrhea Virus

Swine endogenous or exogenous

PEDV

RNA virus

M gene or N gene

Serum, plasma, intestinal tissue, intestinal contents, mesenteric lymph nodes, excreta, etc.

FAM; VIC

5

Porcine Reproductive and Respiratory Syndrome Virus Type North America

Swine endogenous or exogenous

PRRSV

RNA virus

M gene

Lungs, spleen, kidneys, tonsils, lymph, saliva, blood and semen, etc.

FAM; VIC

5

Classical and Variant Strains of Porcine Epidemic Diarrhea Virus Multiplex

Exogenous

PEDV-U+V

RNA virus

Classic strain N and variant S gene

Serum, plasma, intestinal tissue, intestinal contents, mesenteric lymph nodes, excreta, etc.

FAM; VIC; ROX

5

Porcine Reproductive and Respiratory Syndrome Virus Type North America and Highly Pathogenic Variant Strain Multiplex

Exogenous

PRRSV-U+V

RNA virus

Classic strain M and variant NSP2 gene

Lungs, spleen, kidneys, tonsils, lymph, saliva, blood and semen, etc.

FAM; VIC; ROX

5

Classical Swine Fever Virus

Endogenous or Exogenous

CSFV

RNA virus

Polysaccharide protein gene

Tonsils, lymph nodes and other tissue lesions

FAM; VIC

1

Porcine Epidemic Diarrhea Virus, Porcine Transmissible Gastroenteritis Virus and Porcine Rotavirus Group A Multiplex

Exogenous

PEDV/TGEV/PoRV-A

RNA virus

M gene of PEDV, M gene of TGEV and NSP5 gene of PoRV-A

Serum, plasma, intestinal tissue, intestinal contents, mesenteric lymph nodes, excreta, etc.

FAM; VIC; CY5; ROX

5

Porcine Circovirus Type 2

Swine endogenous or exogenous

PCV-2

DNA virus

CAP capsid protein gene

Lungs, lymph nodes, blood, etc.

FAM; VIC

5

Porcine Circovirus Type 3

Swine endogenous or exogenous

PCV-3

DNA virus

CAP capsid protein gene

Lungs, lymph nodes, blood, etc.

FAM; VIC

5

Porcine Circovirus Type 2,3,4 Multiplex

Swine endogenous or exogenous

PCV-2/3/4

DNA virus

CAP capsid protein gene

Lungs, lymph nodes, blood, etc.

FAM; VIC; ROX; CY5

5

Foot-and-Mouth Disease Virus

Exogenous

FMDV-U

RNA virus

Polysaccharide protein gene

Serum, blister skin, blister fluid, or live O/P fluid, etc.

FAM; VIC

5

Foot-and-Mouth Disease Virus Type O

Exogenous

FMDV-O

RNA virus

VP2 gene

Serum, blister skin, blister fluid, or live O/P fluid, etc.

FAM; VIC

5

Foot-and-Mouth Disease Virus Type A

Exogenous

FMDV-A

RNA virus

VP1 gene

Serum, blister skin, blister fluid, or live O/P fluid, etc.

FAM; VIC

5

Foot-and-Mouth Disease Virus Type Asian I

Exogenous

FMDV-Asia I

RNA virus

VP1 gene

Serum, blister skin, blister fluid, or live O/P fluid, etc.

FAM; VIC

5

Porcine Pseudorabies Virus (Gh Gene)

Swine endogenous or exogenous

PRV-gH

DNA virus

National StandardgH gene

Blood, semen, nasal secretions, brain, tonsils, lungs, lymph nodes, etc.

FAM; VIC

2.5

Porcine Pseudorabies Virus (Gb Gene)

Swine endogenous or exogenous

PRV-gB

DNA virus

gB gene

Blood, semen, nasal secretions, brain, tonsils, lungs, lymph nodes, etc.

FAM; VIC

2.5

Porcine Pseudorabies Virus Wild Strain (Ge Gene)

Swine endogenous or exogenous

PRV-gE

DNA virus

gE gene

Blood, semen, nasal secretions, brain, tonsils, lungs, lymph nodes, etc.

FAM; VIC

2.5

Pporcine Pseudorabies Virus Wild Strain and Ge Gene (Gh Gene and Ge Gene) Multiplex

Exogenous or NoInternal Control

PRV-gH+gE

DNA virus

GB gH and gE gene

Blood, semen, nasal secretions, brain, tonsils, lungs, lymph nodes, etc.

FAM; VIC，ROX

2.5

Porcine Pseudorabies Virus Wild Strain and Ge Gene (Gb Gene and Ge Gene) Multiplex

Exogenous or NoInternal Control

PRV-gB+gE

DNA virus

gB and gE gene

Blood, semen, nasal secretions, brain, tonsils, lungs, lymph nodes, etc.

FAM; VIC，ROX

2.5

Porcine Parvovirus Virus

Swine endogenous or exogenous

PPV

DNA virus

VP2 gene

Suspected diseased pig organs, stillbirths and boar semen, etc.

FAM; VIC

2.5

Porcine Delta Coronavirus Virus

Swine endogenous or exogenous

PDcov

RNA virus

M gene

Intestine and intestinal contents, feces, etc.

FAM; VIC

5

Swine Japanese Encephalitis Virus

Swine endogenous or exogenous

JEV

RNA virus

CAP capsid protein gene

Brain tissue, whole blood, etc.

FAM; VIC

1

Swine Vesicular Disease Virus

Swine endogenous or exogenous

SVDV

RNA virus

Polyprotein

Serum, blister skin, blister fluid, or live O/P fluid, etc.

FAM; VIC

5

Erysipelas Suis

Swine endogenous or exogenous

Ery-U

DNA pathogens

Transmembrane protein

Skin rash, joint fluid, endocardial hyperplasia, heart blood, liver, spleen, lymph nodes, etc.

FAM; VIC

5

Erysipelas Suis Wild Strain

Swine endogenous or exogenous

Ery-Y

DNA pathogens

DNA-directed DNA polymerase IV

Skin rash, joint fluid, endocardial hyperplasia, heart blood, liver, spleen, lymph nodes, etc.

FAM; VIC

2.5

Swine Mycoplasma Pneumoniae

Swine endogenous or exogenous

MH

DNA pathogens

16s rRNA

Lung tissue, alveolar lavage fluid, nasal swabs, etc.

FAM; VIC

5

Streptococcus Suis

Swine endogenous or exogenous

SS

DNA pathogens

gdh gene

Swabs, serum or plasma, tonsils, viscera, muscle tissue from raw pigs

FAM; VIC

5

Streptococcus Suis Type 2

Swine endogenous or exogenous

SS-2

DNA pathogens

Cps2J gene

Swabs, serum or plasma, tonsils, viscera, muscle tissue from raw pigs

FAM; VIC

5

Porcine Seneca Virus

Swine endogenous or exogenous

SVA/SVV

RNA virus

P1 protein gene

Pig blistered skin, EDTA anticoagulated whole blood, spleen and other tissues and pig products, etc.

FAM; VIC

1

Haemophilus Parasuis

Swine endogenous or exogenous

HPS

DNA pathogens

infB gene

Blood, no pericardial effusion or pleural effusion or lung tissue, bacterial cultures, etc.

FAM; VIC

2.5

Swine Vesicular Stomatitis Virus

Swine endogenous or exogenous

VSV

RNA virus

N gene

Blistered epithelial tissue, blistered fluid, blood, in the mouth, on the Blistered epithelial tissue, blistered fluid, blood, in the mouth, on the coronet, oral secretions and tissues, etc.

FAM; VIC

5

Porcine Teschovirus

Swine endogenous or exogenous

PTV

RNA virus

polyprotein gene

After pigs are dying or dead, aseptically take heart and blood or liver tissues of sick and dead pigs, etc.

FAM; VIC

2.5

Swine Pasteurellosis

Swine endogenous or exogenous

SPA

DNA pathogens

kmtI gene

Nasal swabs, lung tissue, serum, plasma, etc.

FAM; VIC

5

Influenza A Virus

Exogenous

infA

RNA virus

M gene

Nasal swabs, lung tissue, serum, plasma, etc.

FAM; VIC

5

Swine Influenza Virus Type H1

Exogenous

SIV-H1

RNA virus

HA gene

Nasal swabs, lung tissue, serum, plasma, etc.

FAM; VIC

5

Swine Influenza Virus Type H3

Exogenous

SIV-H3

RNA virus

HA gene

Nasal swabs, lung tissue, serum, plasma, etc.

FAM; VIC

5

Swine Influenza Virus Type H1N1-2009

Exogenous

H1N1-2009

RNA virus

HA gene

Nasal swabs, lung tissue, serum, plasma, etc.

FAM; VIC

5

Swine Actinobacillus Pleuropneumoniae

Exogenous

APP

DNA pathogens

dsbE

Blood, organs with typical lesions such as lungs, hilar lymph nodes, tonsils and nasal secretions

FAM; VIC

5

Porcine Circovirus 4

Exogenous

PCV4

DNA virus

rep gene

Lungs, lymph nodes, blood, etc.

FAM; VIC

2.5

Swine Eperythrozoonosis

Exogenous

Sep

DNA pathogens

A pseudoprotein

Blood

FAM; VIC

5

Swine Lowsonia Intracellularis

Exogenous

LI

DNA pathogens

aspA

Feces, anal swabs, diseased intestinal tissue, etc.

FAM; VIC

2.5

Pathogenic Leptospira

Exogenous

Lin

DNA pathogens

lipL32

Blood, urine, kidney tissue, etc.

FAM; VIC

5

Porcine Reproductive and Respiratory Syndrome Virus Type North America , Highly Pathogenic Variant Strain and Nadc30 Strain Multiplex

Exogenous

PRRSV-U+V+NADC30

RNA virus

Classic strain M and variant NSP2 gene

Lungs, spleen, kidneys, tonsils, lymph, saliva, blood and semen, etc.

FAM; VIC CY5;ROX

5

Porcine Transmissible Gastroenteritis Virus

Exogenous

TGEV

RNA virus

M gene

Serum, plasma, intestinal tissue, intestinal contents, mesenteric lymph nodes, excreta, etc.

FAM; VIC

5

Porcine Rotavirus Group A

Exogenous

PoRV-A

RNA virus

NSP5 gene

Serum, plasma, intestinal tissue, intestinal contents, mesenteric lymph nodes, excreta, etc.

FAM; VIC

5

Porcine Epidemic Diarrhea Virus and Transmissible Gastroenteritis Virus Multiplex

Exogenous

PEDV/TGEV

RNA virus

M gene of PEDV, M gene of TGEV

Serum, plasma, intestinal tissue, intestinal contents, mesenteric lymph nodes, excreta, etc.

FAM; VIC; ROX

5

Porcine Rotavirus Group A and Porcine Delta Coronavirus Virus  Multiplex

Exogenous

PoRV-A/PDCoV

RNA virus

NSP5 gene of PoRV-A and M gene of PDcov

Serum, plasma, intestinal tissue, intestinal contents, mesenteric lymph nodes, excreta, etc.

FAM; VIC; ROX

5

Porcine Reproductive and Respiratory Syndrome Virus Nadc30 Strain

Swine endogenous or exogenous

PRRSV-NADC30

RNA virus

M gene

Lungs, spleen, kidneys, tonsils, lymph, saliva, blood and semen, etc.

FAM; VIC

5

Porcine Reproductive and Respiratory Syndrome Virus Type North America, Porcine Pseudorabies Virus and Classical Swine Fever Virus Multiplex

Exogenous

PRRSV/PRV/CSFV

RNA/DNA virus

Specific conservative gene

Blood, semen, nasal secretions, brain, tonsils, lungs, lymph nodes, etc.

FAM; VIC; ROX

5

Porcine Reproductive and Respiratory Syndrome Virus Type Classical North America and Highly Pathogenic Variant Strain  Multiplex

Exogenous

PRRSV-C+V

RNA virus

NSP2 gene

Lungs, spleen, kidneys, tonsils, lymph, saliva, blood and semen, etc.

FAM; VIC; ROX

5

Porcine Circovirus Type 1,2 Multiplex

Exogenous

TTSuV1/2

DNA virus

Specific conservative gene

Lungs, lymph nodes, blood, etc.

FAM; VIC; ROX

5

Porcine Reproductive and Respiratory Syndrome Virus Type North America, African Swine Fever Virus and Porcine Pseudorabies Virus Multiplex

NoInternal Control

PRRSV/PRV/ASFV

RNA/DNA virus

Specific conservative gene

Oral and nasal swabs, whole blood, tissue, saliva, feed, sewage, excreta and other samples

FAM; VIC; ROX

5

African Swine Fever Virus P72 Gene and I177L Gene

Swine Endogenous

P72+I177L

DNA virus

P72+I177L gene

Oral and nasal swabs, whole blood, tissue, saliva, feed, sewage, excreta and other samples

FAM; VIC; ROX

5

Porcine Reproductive and Respiratory Syndrome Virus Type North America, Classical Swine Fever Virus, Porcine Pseudorabies Virus and Porcine Circovirus Type 2 Multiplex

NoInternal Control

PRRSV/PRV/CSFV/PCV2

RNA/DNA virus

Specific conservative gene

Blood, semen, nasal secretions, brain, tonsils, lungs, lymph nodes, etc.

FAM; VIC; CY5; ROX

5

Isospora and Eimeria(Multiplex  Fluorescence PCR)

Swine Endogenous

Iso/Eim

DNA pathogens

Specific conservative gene

Feces

FAM; VIC; ROX

5

Porcine Reproductive and Respiratory Syndrome Virus Type Classical North America(Fluorescence RT-PCR)

Exogenous

PRRSV-U

RNA virus

NSP2 gene

Lungs, spleen, kidneys, tonsils, lymph, saliva, blood and semen, etc.

FAM; VIC

5